| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Winfield MS | A | Penrod | Lewis | Seidel | Burk | 4:55.27 | Verified |
| 2 | Wellsville-Middletown Middle Sch | A | Bickell | Marshall | Norris | Slovensky | 4:26.19 | Verified |
| 3 | Elsberry Middle School | A | Santos | Schrader | Hausgen | Twellman | 4:22.06 | Verified |
| 4 | North Callaway MS | A | Cunningham | Drake | Kemp | Moore | 4:25.65 | Verified |
| 5 | Louisiana Middle School | A | Mayfield | Sheppard | Stoops | Wilkinson | 4:37.70 | Verified |
| 6 | Clopton Middle School | A | Salmons | Eivins | Jennewein | Alexander | 4:57.00 | Verified |
