Section 1 of 2
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Elsberry Middle School | A | Santos | Underwood | Lynch | Edwards | 1:00.48 | Verified |
| 3 | Wellsville-Middletown Middle Sch | A | Bickell | Henderson | Marshall | Slovensky | 57.61 | Verified |
| 4 | Van-Far MS | A | Hays | Fischer | Utterback | Christian | 58.75 | Verified |
Section 2 of 2
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Clopton Middle School | A | Hall | Matheny | Matheny | Evans | 54.64 | Verified |
| 3 | Winfield MS | A | Barth | Colbert | Martin | Morse | 51.28 | Verified |
| 4 | North Callaway MS | A | Blair | Cunningham | Green | Lee | 53.80 | Verified |
| 5 | Louisiana Middle School | A | Mayfield | McCarty | Stoops | Wilkinson | 56.14 | Verified |
