| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Clopton Middle School | A | Adam | Williamson | Graham | Mitchell | 1:02.65 | Verified |
| 2 | Elsberry Middle School | A | Howard | Lovelace | Smith | Talbot | 59.50 | Verified |
| 3 | Winfield MS | A | VanCleve | Nelke | Reinebach | Reinebach | 55.28 | Verified |
| 4 | Louisiana Middle School | A | Pedersen | Ries | Wallace | McGlothin | 55.68 | Verified |
| 5 | North Callaway MS | A | Bedsworth | Horstman | Jones | Smithee | 59.51 | Verified |
| 6 | Van-Far MS | A | Brogden | Cashman | Kampeter | Heaton | 1:05.28 | Verified |
