| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | North Callaway MS | A | Wine | Nevels | Higgins | Minor | 2:02.77 | Verified |
| 2 | Van-Far MS | A | Green | Gaston | Hays | Utterback | 1:57.72 | Verified |
| 3 | Winfield MS | A | Burk | Johnson | Moore | Dillin | 1:52.84 | Verified |
| 4 | Louisiana Middle School | A | Blackwell | Love | Sheppard | Nichtula | 1:53.50 | Verified |
| 5 | Elsberry Middle School | A | Heras | Byers | Underwood | Santos | 2:01.89 | Verified |
| 6 | Clopton Middle School | A | Hall | Salmons | Eivins | Alexander | 2:08.14 | Verified |
