| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | North Callaway MS | A | Jones | Miles | Smithee | Bedsworth | 2:17.96 | Verified |
| 2 | Winfield MS | A | Rau | Pund | Ford | Meyer | 2:07.55 | Verified |
| 3 | Elsberry Middle School | A | Talbot | Howard | Hartley | Talbot | 1:59.78 | Verified |
| 4 | Louisiana Middle School | A | Wommack | Perrine | Lentz | Lesley | 2:03.08 | Verified |
| 5 | Clopton Middle School | A | Bankhead | Williamson | Graham | Mitchell | 2:13.31 | Verified |
| 6 | Van-Far MS | A | Cashman | Grinde | Kampeter | Motley | 2:25.45 | Verified |
