| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Missouri Military Academy | A | Beverido | Salinas | Ruckman | Wever | 3:58.34 | Verified |
| 2 | Mark Twain High School | A | Lake | Lake | Owen | McLeod | 3:50.67 | Verified |
| 3 | Bowling Green High School | A | Hearn | Griffith | Gamm | Starks | 3:47.22 | Verified |
| 4 | Winfield High School | A | Hyde | Mense | Sams | Carson | 3:50.28 | Verified |
| 5 | Wellsville-Middletown High Schoo | A | Mayes | Moore | Seabaugh | Hollensteiner | 3:51.88 | Verified |
| 6 | Van-Far High School | A | Kliewer | Gibson | Lay | Caldwell | 3:59.97 | Verified |
