| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WRIGHT, Trinity | SO | Highland High School | 2:37.64 | Verified |
| 2 | THOROUGHMAN, Rita | SO | Silex High School | 2:46.73 | Verified |
| 3 | KLOTT, Rachel | 9 | Bowling Green High School | 2:47.72 | Verified |
| 4 | BRYAN, Alayna | JR | Van-Far High School | 2:50.75 | Verified |
| 5 | HARSHBERGER, Emma | 9 | Highland High School | 2:55.31 | Verified |
| 6 | BOECKELMAN, Jenelle | 9 | Wellsville-Middletown High Schoo | 2:55.72 | Verified |
| 7 | TREDWAY, Ashlynn | 9 | Louisiana High School | 3:00.71 | Verified |
| 8 | SUTTON, Katelynn | 9 | Van-Far High School | 3:13.01 | Verified |
| 9 | LINDSAY, Carly | 9 | Silex High School | 3:16.46 | Verified |
| 10 | WELLS, Natalee | 9 | Bowling Green High School | 3:19.23 | Verified |
| 11 | MYERS, Faith | SR | Clopton High School | 3:19.38 | Verified |
| 12 | SCHLEEPER, Madison | 9 | Winfield High School | 3:21.03 | Verified |
| 13 | INCE, Alexis | JR | Louisiana High School | 3:24.65 | Verified |
| 14 | GERHOLDT, Madelyn | SO | Winfield High School | 3:31.03 | Verified |
| 15 | WEITKAMP, Macy | JR | Clopton High School | 3:31.67 | Verified |
| 16 | MACKEY, Paige | 9 | Winfield High School | 3:34.49 | Verified |
| 17 | RESOR, Cynthia | 9 | Mark Twain High School | NT | Unverified: |
| 18 | CARROLL, Kortney | 9 | Bowling Green High School | NT | Unverified: |
| 19 | BOOTH, Lauren | SO | Mark Twain High School | NT | Unverified: |
