| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Wellsville-Middletown High Schoo | A | Scott | Forir | Jefferson | Boeckelman | 59.77 | Verified |
| 2 | Louisiana High School | A | Campbell | Chatman | Jenkins | Sencenbaugh | 56.34 | Verified |
| 3 | Highland High School | A | Abell | Bringer | Goings | Reed | 54.19 | Verified |
| 4 | Mark Twain High School | A | Armour | Black | Boleach | Ross | 55.20 | Verified |
| 5 | Silex High School | A | Enos | Thoroughman | Kuntz | Hall | 57.89 | Verified |
| 6 | Clopton High School | A | Akers | Lucas | Myers | Denslow | 1:01.33 | Verified |
