| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Wellsville-Middletown High Schoo | A | Mayes | Norris | Seabaugh | Peak | 9:16.12 | Verified |
| 2 | Mark Twain High School | A | Black | Lake | McLeod | Owen | 9:22.11 | Verified |
| 3 | Winfield High School | A | Trempler | Kaimann | Harl | Geiss | 10:02.07 | Verified |
| 4 | Van-Far High School | A | Gibson | Lay | Childs | Caldwell | 10:10.18 | Verified |
| 5 | Missouri Military Academy | A | Salinas | Ruckman | Kazoora | Kagame | 10:34.27 | Verified |
