| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Marshall | A | Keller | Wilson | McFarland | Scott | 56.00 | |
| 3 | Olney (Richland County) | A | Beal | Klier | Bloomer | Ingram | 54.10 | |
| 4 | Flora | A | Young | Rodriguez | Scott | Peterman | 53.90 | |
| 5 | Bridgeport (Red Hill) | A | Hulfachor | Fisher | Hasewinkle | Hasewinkle | 53.97 | |
| 6 | Robinson | A | Zane | Blank | Treadway | Weber | 54.90 | |
| 7 | Newton | A | Zumbahlen | Bennett | Schafer | Russell | 57.34 |
