| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Bridgeport (Red Hill) | A | Hasewinkle | Loudermilk | Klein | Loudermilk | 4:53.44 | |
| 2 | Marshall | A | Gard | Arthur | Sanders | Wilson | 4:45.00 | |
| 3 | Casey (C.-Westfield) | A | Curry | Ross | Self | Wright | 4:32.00 | |
| 4 | Robinson | A | Zane | Blank | Zane | Weber | 4:18.90 | |
| 5 | Newton | A | Bennett | Borgic | Hemrich | Zumbahlen | 4:20.00 | |
| 6 | Flora | A | Young | Rodriguez | Scott | Peterman | 4:33.00 | |
| 7 | Olney (Richland County) | A | Greenwood | Harness | Greenwood | Clodfelter | 4:47.26 |
