| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | TRAUB, Eli | SO | Flora | 1:01.88 | |
| 2 | GILMORE, Noah | FR | Robinson | 56.44 | |
| 3 | JONES, Saul | SO | Olney (Richland County) | 54.12 | |
| 4 | WOLF, Parker | FR | Newton | 52.66 | |
| 5 | SCHAFER, Evan | JR | Newton | 52.84 | |
| 6 | RODRIGUEZ, Alex | JR | Flora | 56.04 | |
| 7 | HERRERA, Vicente | JR | Casey (C.-Westfield) | 56.84 | |
| 8 | HARMON, Jackson | SO | Robinson | 1:06.34 |
