| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Casey (C.-Westfield) | A | Bohner | Crutchfield | Herrera | Holmes | 1:54.00 | |
| 2 | Flora | A | Gibson | Redenbo | Closson | Propes | 1:41.20 | |
| 3 | Marshall | A | McFarland | Maxwell | Rees | Boyer | 1:39.40 | |
| 4 | Robinson | A | Johnson | York | Jackson | Peternel | 1:35.50 | |
| 5 | Newton | A | Meinhart | Tarr | Flowers | Zavala | 1:37.41 | |
| 6 | Olney (Richland County) | A | Runyon | Lathrop | Hough | Piercefield | 1:40.00 | |
| 7 | Paris | A | Decker | Philips | Rigdon | Atkinson | 1:43.00 |
