| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Flora | A | Pickens | Smith | Leib | Rodriguez | 2:07.60 | |
| 3 | Robinson | A | Elder | Blank | Treadway | Wesley | 1:56.10 | |
| 4 | Bridgeport (Red Hill) | A | Hulfachor | Loudermilk | Hasewinkle | Hasewinkle | 1:54.11 | |
| 5 | Newton | A | Zumbahlen | Schafer | Bennett | Russell | 1:55.00 | |
| 6 | Olney (Richland County) | A | Greenwood | Burgener | Brown | Greenwood | 2:01.30 |
