| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Metro High School | A | Carter | Custis | Gondzur | Dawson | NT | Verified |
| 2 | Gateway High School | A | DQ | Verified | ||||
| 4 | Trinity Catholic High School | A | Hibbler | Oliphant | Cannon | Juszczyk | 1:31.10 | Verified |
| 5 | Cardinal Ritter High School | A | 1:33.16 | Verified | ||||
| 6 | Westminster Christian Academy | A | Hines | Parks | Mulick | Wilkerson | 1:37.06 | Verified |
| 7 | Riverview Gardens High School | A | Davis | Abel | Hodges | Howard | 1:41.55 | Verified |
