| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Lift for Life Academy Charter Hi | A | Hall | Seals | Williams | Mills | 1:56.66 | Verified |
| 3 | Riverview Gardens High School | A | Mccarter | Richardson | Shelton | GLADDEN | 1:53.45 | Unverified: |
| 4 | Cardinal Ritter High School | A | 1:42.57 | Verified | ||||
| 5 | Incarnate Word Academy | A | 1:52.50 | Verified | ||||
| 6 | Trinity Catholic High School | A | 1:56.24 | Verified | ||||
| 7 | Metro High School | A | Christian | Fuchs | Kamara | Macon-Ford | 2:02.96 | Verified |
