| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Lift for Life Academy Charter Hi | A | Gillespie | Seals | Hardieway | Williams | NT | Verified |
| 3 | Riverview Gardens High School | A | Mccarter | Richardson | Zimmerman | GLADDEN | NT | Unverified: |
| 4 | Cardinal Ritter High School | A | 4:04.15 | Verified | ||||
| 5 | Westminster Christian Academy | A | Draper | Bradley | Shively | Beachy | 4:35.70 | Verified |
| 6 | Trinity Catholic High School | A | NT | Verified | ||||
| 7 | Metro High School | A | Baum-Scholle | Leonard | Anania | Grimes | NT | Verified |
