| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BAUM-SCHOLLE, Lucy | 9 | Metro High School | NT | Unverified: |
| 2 | DAVIS, Haley | 9 | Incarnate Word Academy | 57.66 | Verified |
| 3 | GREGORY, Cadyn | 9 | Cardinal Ritter High School | 50.96 | Verified |
| 4 | WALLACE, hannah | 9 | Cardinal Ritter High School | 49.63 | Verified |
| 5 | GRAY, Hannah | JR | Trinity Catholic High School | 50.07 | Verified |
| 6 | CADE, Kimmeran | JR | Metro High School | 51.90 | Verified |
| 7 | WITHERSPOON, Bria | SO | Westminster Christian Academy | 1:02.96 | Verified |
