| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Metro High School | A | Hudson | Fuchs | Kamara | Macon-Ford | 57.42 | Verified |
| 2 | Westminster Christian Academy | A | Scheulen | Phillips | Schofield | Bradley | 54.62 | Verified |
| 3 | Incarnate Word Academy | A | 52.43 | Verified | ||||
| 4 | Cardinal Ritter High School | A | 48.62 | Verified | ||||
| 5 | Trinity Catholic High School | A | 51.24 | Verified | ||||
| 6 | Lift for Life Academy Charter Hi | A | Gillespie | Mills | Seals | Williams | 53.87 | Verified |
| 7 | Riverview Gardens High School | A | Mccarter | Richardson | Shelton | Zimmerman | 54.78 | Unverified: Parkway North Girls Invitational |
