| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | SIU Edwardsville | A | Brown | Bretz | McCarthy | Nuzzo | 41.71 | 2019 SIUE Gateway Classic |
| 4 | Memphis | A | Whitehead | West II | Vance | Jackson | 39.19 | NCAA Division I East Region Preliminary Rounds |
| 5 | Chicago State | A | Spells | Cesalien | Keith | Jackson | 41.65 | |
| 6 | Tennessee-Martin | A | Hayes | Martin | Holland | Smith | 42.58 | Ole Miss Classic |
