| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Chicago State | B | Pulliam-Robinson | Ataessien | Chambers | Patterson | 49.50 | |
| 2 | Chicago State | A | Jackson | Nelson | Blackwell | Criswell | DNF | |
| 3 | Memphis | A | Brown | Cross | Booker | Perkins | 46.69 | Al Schmidt Bulldog Relays Invite |
| 4 | Murray State | A | Denning | James | Herrero-Yanez | Hagans | 45.34 | Margaret Simmons Invite |
| 5 | Missouri State | A | Weaver | Meredith | Askew | Henry | 45.86 | Tom Botts Invitational |
| 6 | SE Missouri | A | Cogio | Miles | James | Williams | NT | |
| 7 | Western Illinois | A | Peterson | Taylor | Harrison | Scott | NT | Mizzou Spring Open |
| 8 | Missouri State | B | King | Richey | Gray | Anderson | 50.45 |
