| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | WATENE, Wambui | SR | Murray State | 1:07.76 | Margaret Simmons Invite |
| 3 | DIEBENOW, Michella | FR | Belmont | 1:06.75 | Margaret Simmons Invite |
| 4 | LONG, Kendall | JR | SE Missouri | 1:00.61 | OVC Championship |
| 5 | PRUITT, Caroline | SR | Belmont | 1:03.42 | OVC Championship |
| 6 | HITCHCOCK, Tara | SO | Belmont | 1:07.43 | Margaret Simmons Invite |
| 7 | KARNEY, Sophie | FR | Missouri State | NT |
