Section 1 of 2
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Chaminade College Preparatory Sc | A | Cameron) Epps | Cavlovich | Lee | Johnson | NT | Verified |
| 2 | St. Louis University High School | A | Chaffee | Bailey | Parker | Greninger | 1:36.20 | Unverified: |
| 3 | Riverview Gardens High School | A | Abel | Davis | Hodges | Love | NT | Unverified: |
| 4 | DeSmet Jesuit High School | A | Hentges | Kirisits | Haddock | Sharp | 1:36.10 | Verified |
| 5 | Christian Brothers College High | A | Smith | Martin | Conley | McClellan | 1:36.40 | Verified |
| 6 | Kirkwood High School | A | Welch | Malone | Smith | Harris | 1:40.00 | Unverified: |
Section 2 of 2
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Oakville High School | A | Abuzaid | BRADLEY | REDFORD | MUSTAJBEGOVIC | 1:46.80 | Verified |
| 3 | Priory High School | A | Singer | Schnurbusch | Roxas | Mercer | 1:40.00 | Unverified: |
| 4 | Northwest (Cedar Hill) High Scho | A | Robinson | Grellner | Vandiver | Ash | 1:41.60 | Verified |
| 5 | Vianney High School | A | 1:54.70 | Verified |
