Section 1 of 2
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Kirkwood High School | A | Welch | Malone | Smith | Harris | 47.00 | Unverified: |
| 2 | St. Louis University High School | A | Greninger | Parker | Bailey | Chaffee | 46.27 | Unverified: |
| 3 | Christian Brothers College High | A | Woods | Martin | McClellan | Love | 43.57 | Unverified: Holt Invitational |
| 4 | DeSmet Jesuit High School | A | Stewart | Gray | Thomas | Cloud | DQ | Verified |
| 5 | Chaminade College Preparatory Sc | A | Cameron) Epps | Gray | Johnson | Lee | 46.83 | Verified |
| 6 | Riverview Gardens High School | A | Hodges | Howard | Love | Abel | NT | Unverified: |
Section 2 of 2
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Vianney High School | A | 52.34 | Verified | ||||
| 3 | Priory High School | A | Singer | Glarner | McMillan | Mercer | 47.70 | Unverified: |
| 4 | Northwest (Cedar Hill) High Scho | A | Robinson | Grellner | Vandiver | Brandt | 48.11 | Verified |
