| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Red Bud | A | Wolter | Sievers | Pensoneau | Mulherin | 2:09.84 | |
| 2 | Breese (Mater Dei) | A | Brokering | Strubhart | Zurliene | Toennies | 2:04.24 | |
| 3 | Trenton (Wesclin) | A | Bernreuter | Bernreuter | Litteken | Williams | 2:00.35 | |
| 4 | Nashville | A | Thorson | Reynolds | Kozuszek | Rueter | 1:48.17 | |
| 5 | Belleville (Althoff Catholic | A | Douglas | Cange | Conrad | Lester | 1:54.14 | |
| 6 | Madison | A | Spencer | Bursey | Gardner | LEE | 2:00.49 | |
| 7 | Alton (Marquette) | A | Certa | Sanders | Rister | Rae | 2:08.74 | |
| 8 | Okawville | A | Sweet | Wood | Rennegarbe | Bening | 2:13.94 |
