| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Alton (Marquette) | A | Fraley | Certa | Sanders | Rae | DNS | |
| 2 | Trenton (Wesclin) | A | Litteken | Bernreuter | Bernreuter | Schulte | 55.27 | |
| 3 | Belleville (Althoff Catholic | A | Hebel | Cange | Leatherman | Conrad | 53.54 | |
| 4 | Nashville | A | Thorson | Reynolds | Kozuszek | Rueter | 50.55 | |
| 5 | Madison | A | Bursey | Belford | Gardner | LEE | 53.36 | |
| 6 | Dupo | A | Ellis | Jordan | Houston | Heidleberg | 54.64 | |
| 7 | Okawville | A | Snyder | Luechtefeld | Wolf | Haacke | 55.47 | |
| 8 | Breese (Mater Dei) | A | Brokering | Strubhart | Fuehne | Toennies | 57.26 |
