| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Trenton (Wesclin) | A | Huffman | Wesselmann | Williams | McAllister | 5:03.22 | |
| 3 | Okawville | A | Snyder | Luechtefeld | Buehler | Haacke | 4:44.74 | |
| 4 | Belleville (Althoff Catholic | A | Douglas | Conrad | Lester | Parks | 4:23.91 | |
| 5 | Nashville | A | Newman | Heggemeier | Matecki | Baugh | 4:27.20 | |
| 6 | Alton (Marquette) | A | Johnson | Vickrey | Rister | Certa | 4:48.84 | |
| 7 | Red Bud | A | Piel | Braun | Vallett | Pensoneau | 5:05.24 |
