Section 1 of 3
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CONRAD, Caitlin | JR | Belleville (Althoff Catholic | NT | |
| 2 | RAINEY, Clara | FR | Edwardsville (Metro-East Lut | 34.84 | |
| 3 | SCHNEIDER, Hadley | SO | Waterloo (Gibault Catholic) | 32.34 | |
| 4 | BRAMMEIER, Mikayla | SR | Centralia (Christ Our Rock L | 31.34 | |
| 5 | ZEEB, Destiny | SO | Lebanon | 32.04 | |
| 6 | LOPEZ, Erica | FR | Trenton (Wesclin) | 33.56 | |
| 7 | WILSON, Baylee | FR | New Athens | 35.44 |
Section 2 of 3
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SCHULTE, Maddie | FR | Trenton (Wesclin) | 31.14 | |
| 2 | CARTER, Krista | JR | Centralia (Christ Our Rock L | 30.90 | |
| 3 | WOLF, Maci | SO | Okawville | 30.34 | |
| 4 | HEIDLEBERG, Octavia | JR | Dupo | 30.24 | |
| 5 | SPENCER, Kaleigh | FR | Madison | 30.31 | |
| 6 | BALDRIDGE, Briana | FR | Waterloo (Gibault Catholic) | 30.54 | |
| 7 | GARDNER, Armya | FR | Madison | 31.03 | |
| 8 | RAE, Caroline | SO | Alton (Marquette) | 31.34 |
Section 3 of 3
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BROKERING, Trina | SR | Breese (Mater Dei) | 28.44 | |
| 2 | HOFFARTH, Lidiah | FR | New Athens | 28.00 | |
| 3 | HOUSTON, Kabrina | JR | Dupo | 27.34 | |
| 4 | KOZUSZEK, Talanie | JR | Nashville | 26.66 | |
| 5 | PARKS, Nariah | SR | Belleville (Althoff Catholic | 26.74 | |
| 6 | REYNOLDS, Karmen | SR | Nashville | 27.74 | |
| 7 | HENTRICH, Sammy | SO | Alton (Marquette) | 28.14 | |
| 8 | ZURLIENE, Kayla | SO | Breese (Mater Dei) | 29.54 |
