Section 1 of 2
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | HEBEL, Kennadi | SO | Belleville (Althoff Catholic | 57.88 | |
| 4 | SWEET, Brianna | FR | Okawville | 1:10.14 | |
| 5 | ZEEB, Destiny | SO | Lebanon | 1:00.24 |
Section 2 of 2
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | DOUGLAS, Azia | JR | Belleville (Althoff Catholic | 55.44 | |
| 3 | HENTRICH, Sammy | SO | Alton (Marquette) | 52.34 | |
| 4 | LUECHTEFELD, Lydia | FR | Okawville | 51.14 | |
| 5 | VANHISE, Emma | SO | Nashville | 51.79 | |
| 6 | ELLIS, Allyson | SR | Dupo | 52.74 | |
| 7 | FINKE, Daci | SO | Nashville | 55.45 |
