Section 1 of 2
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FUEHNE, Evelyn | FR | Breese (Mater Dei) | NT | |
| 2 | LIEFER, Simone | SO | Red Bud | 1:27.53 | |
| 3 | SCHNEIDER, Hadley | SO | Waterloo (Gibault Catholic) | 1:16.44 | |
| 4 | BUEHLER, Kylie | SO | Okawville | 1:14.53 | |
| 5 | REILMANN, Lilly | FR | Trenton (Wesclin) | 1:15.53 | |
| 6 | MUSCARELLO, Sam | FR | Breese (Mater Dei) | 1:17.74 | |
| 7 | SIEGFRIED, Chloe | SO | Trenton (Wesclin) | NT |
Section 2 of 2
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BAUGH, Hailey | JR | Nashville | 1:06.02 | |
| 2 | VICKREY, Kailey | SO | Alton (Marquette) | 1:05.53 | |
| 3 | BALDRIDGE, Briana | FR | Waterloo (Gibault Catholic) | 1:04.24 | |
| 4 | PARKS, Nariah | SR | Belleville (Althoff Catholic | 58.54 | |
| 5 | HAACKE, Lauren | FR | Okawville | 1:03.17 | |
| 6 | NEWMAN, Bailey | SO | Nashville | 1:04.34 | |
| 7 | HOFFARTH, Lidiah | FR | New Athens | 1:05.94 | |
| 8 | ARNOLD, Sierra | SO | Patoka | 1:07.14 |
