| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Raymore-Peculiar High School | A | NT | |||||
| 3 | Blue Springs South High School | A | Macias | Sauni | Van Wyk | Kochman | 53.00 | Unverified: |
| 4 | Blue Springs High School | A | NT | Unverified: | ||||
| 5 | Grain Valley High School | A | McAnerney | Winter | Perkins | Gallagher | 51.84 | Unverified: |
| 6 | North Kansas City High School | A | Beltran | Cosentino | Baehr | Joiner | NT | Unverified: |
