| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Fort Osage High School | A | Estepan | Shepherd | Parsons | Velder | 3:47.86 | Verified |
| 2 | Blue Springs South High School | A | Bausby | Dowler | Fournier | Seymour | 3:37.76 | Verified |
| 3 | Lee's Summit West High School | A | 3:29.74 | Verified | ||||
| 4 | Grain Valley High School | A | Hart | Vrlenich | Pratt | Keller | 3:27.47 | Verified |
| 5 | Blue Springs High School | A | 3:28.10 | Verified | ||||
| 6 | Rockhurst High School | A | Tharp | Hill | Stoltz | Campbell | 3:33.91 | Verified |
| 7 | Ruskin High School | A | WILLIAMS | Assiongbon | Dewberry | Holley Nelson | 3:41.69 | Verified |
