| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Cuba High School | A | Chase | Dake | Hinojosa | Nelson | 4:03.62 | Verified |
| 4 | Steelville High School | A | Brown | Merseal | Sutton | Diaz | 3:48.96 | Verified |
| 5 | Vienna High School | A | Horman | Casey | Magner | Coons | 3:56.07 | Verified |
