| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Vienna High School | A | Terwilliger | Veasman | Fick | Murphy | 5:25.84 | Verified |
| 4 | Steelville High School | A | Diaz | Woodall | Evans | Pryor | 4:24.15 | Verified |
| 5 | Cuba High School | A | Black | Herr | Ijames | Moreland | 4:56.81 | Verified |
