| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SHARP, Theadora | 9 | Cuba High School | NT | Unverified: |
| 2 | NOVAK, Madeleine | SO | Vienna High School | 1:07.84 | Verified |
| 3 | DELUNA, Alejandra | JR | Cuba High School | 1:04.47 | Verified |
| 4 | DAVIS, Lauren | JR | Steelville High School | 53.08 | Verified |
| 5 | KREITNER, Abby | 9 | Steelville High School | 57.55 | Verified |
| 6 | EVANS, Norah | 9 | Steelville High School | 1:05.00 | Unverified: |
| 7 | SILVA, Cadance | JR | Cuba High School | 1:13.75 | Verified |
