| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Cuba High School | A | Brown | Nelson | Scott | Minardi | 49.28 | Verified |
| 4 | Steelville High School | A | Frisk | Brown | Killeen | Franz | 48.56 | Verified |
| 5 | Vienna High School | A | Casey | Magner | Coons | Kleffner | 48.94 | Verified |
| 6 | Belle High School | A | Smith | Hoehne | Butler | Kottwitz | NT | Verified |
