| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Vienna High School | A | Schulte | Rosentreter | Veasman | Novak | 58.54 | Verified |
| 4 | Steelville High School | A | Perkins | Davis | Booker | Cottrell | 55.11 | Verified |
| 5 | Cuba High School | A | Dong | Moreland | Henke | Mercer | 56.71 | Verified |
