| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DIAZ, Courtney | SO | Steelville High School | 5:59.15 | Verified |
| 2 | FULLIAM, Kaylee | 9 | Cuba High School | 6:08.87 | Verified |
| 3 | TURNBOUGH, Audrey | 9 | Steelville High School | 6:26.81 | Verified |
| 4 | BROWN, Melayna | JR | Cuba High School | 6:31.57 | Verified |
| 5 | VIRAMONTES, Annia | 9 | Cuba High School | 7:43.69 | Verified |
| 6 | MURPHY, Danielle | SO | Vienna High School | 7:52.09 | Verified |
