| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Cuba High School | A | Black | Brooks | Scott | Hulsey | 1:50.37 | Verified |
| 4 | Vienna High School | A | Andrews | Coons | Casey | Kleffner | 1:36.59 | Verified |
| 5 | Steelville High School | A | Brown | Harris | Killeen | Franz | 1:40.52 | Verified |
| 6 | Belle High School | A | Kottwitz | Hoehne | Butler | Smith | NT | Verified |
