| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Vienna High School | A | Novak | Terwilliger | Rosentreter | Veasman | 2:09.90 | Verified |
| 4 | Steelville High School | A | Perkins | Cottrell | Pryor | Davis | 1:56.94 | Verified |
| 5 | Cuba High School | A | Herr | Ijames | Deluna | Dong | 2:03.43 | Verified |
