| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | St. Charles High School | A | Coffman | Schiffer | Rayburn | Nash | 4:44.62 | Verified |
| 4 | St. Charles West High School | A | Myers | Zeier | Malinee | Nichols | NT | Verified |
| 5 | Winfield High School | A | Turnbull | Rudar | Berry | Kukan | 4:37.54 | Verified |
| 6 | Orchard Farm High School | A | Bauer | Schwierjohn | Barnes | Janson | NT | Verified |
