| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JAMES, Elizabeth | 9 | Warrenton High School | 36.91 | Verified |
| 2 | BAER, Matilynn | SO | Orchard Farm High School | 36.21 | Verified |
| 3 | BICKEL, Antonia | JR | Warrenton High School | 34.35 | Verified |
| 4 | CROSS, Abigail | 9 | St. Charles High School | 32.64 | Verified |
| 5 | RUDLOFF, Taylor | 9 | Orchard Farm High School | 33.09 | Verified |
| 6 | SWINK, Jayla | 9 | Orchard Farm High School | 34.70 | Verified |
| 7 | MARTIN, Allyson | SO | St. Charles West High School | 36.31 | Verified |
| 8 | HOLLRAH, Jenna | 9 | St. Charles High School | NT | Unverified: |
