| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JACKSON, Lilian | JR | St. Charles West High School | 2:24.39 | Verified |
| 2 | KUKAN, Kiley | 9 | Winfield High School | 2:29.64 | Verified |
| 3 | MARSCHEL, Madelyn | SO | Warrenton High School | 2:36.18 | Verified |
| 4 | GROOMS, Brianna | 9 | Warrenton High School | 2:44.40 | Verified |
| 5 | GILKISON, Emma | SO | St. Charles High School | 2:45.57 | Verified |
| 6 | EVERLEY, Taylor | JR | St. Charles West High School | 2:56.22 | Verified |
| 7 | HULBERT, Emma | SR | Orchard Farm High School | 2:57.10 | Verified |
| 8 | CATTOOR, Lily | SR | Orchard Farm High School | 2:58.82 | Verified |
| 9 | RAYBURN, Jonni | JR | St. Charles High School | 3:03.68 | Verified |
| 10 | SCHLEEPER, Madison | 9 | Winfield High School | 3:12.25 | Verified |
