| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | BOURGEADE, Luc | SO | St. Charles High School | 47.34 | Verified |
| 3 | HYDE, Matthew | 9 | Winfield High School | 44.99 | Verified |
| 4 | BALDWIN, Xavier | JR | Winfield High School | 43.14 | Verified |
| 5 | MERTENS, Nicholas | SR | Warrenton High School | 44.37 | Verified |
| 6 | NENNERT, Charles | JR | St. Charles High School | 46.57 | Verified |
| 7 | REINEKE, James | JR | Orchard Farm High School | 56.05 | Verified |
