| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SCHWIERJOHN, Lauryn | 9 | Orchard Farm High School | NT | Unverified: |
| 2 | BAUER, Emma | JR | Orchard Farm High School | 1:10.33 | Verified |
| 3 | BESSER, Veronika | SO | Warrenton High School | 1:07.56 | Verified |
| 4 | RUDAR, Jessi | SO | Winfield High School | 1:06.87 | Verified |
| 5 | MYERS, Makayla | SO | St. Charles West High School | 1:07.45 | Verified |
| 6 | ZEIER, Alivia | 9 | St. Charles West High School | 1:09.22 | Verified |
| 7 | COFFMAN, Te'llah | JR | St. Charles High School | 1:12.19 | Verified |
| 8 | SCHIFFER, Isabella | SR | St. Charles High School | NT | Unverified: |
