| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Charles High School | A | Marberry | Newberry | Calmese | Walker | NT | Unverified: |
| 3 | Warrenton High School | A | Evans | Reel | Parker | Meine | 45.94 | Verified |
| 4 | Winfield High School | A | Baldwin | Sams | Mense | Carson | 44.44 | Verified |
| 5 | Orchard Farm High School | A | West | Williams | Lewis | Snow | 44.50 | Verified |
| 6 | St. Charles West High School | A | Jiles | Foster | LeBeau | Smith | 46.28 | Verified |
