| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Warrenton High School | A | DeHart | Schneider | Pruitt | Besser | 54.40 | Verified |
| 4 | St. Charles West High School | A | House | Shivers | Watson | Nichols | DQ | Verified |
| 5 | Winfield High School | A | Zellmer | Davidson | Rudar | Bradshaw | DQ | Verified |
| 6 | St. Charles High School | A | Kinner | Long | Oellermann | Starks | 54.64 | Verified |
