| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JACKSON, Lilian | JR | St. Charles West High School | 5:11.95 | Verified |
| 2 | BERRY, Kade | SO | Winfield High School | 5:38.36 | Verified |
| 3 | JACKSON, Avery | 9 | St. Charles West High School | 5:59.59 | Verified |
| 4 | GROOMS, Brianna | 9 | Warrenton High School | 6:27.80 | Verified |
| 5 | BOSCHERT, Lily | SO | Winfield High School | 6:39.84 | Verified |
| 6 | HOWARD, Loren | 9 | Warrenton High School | 6:42.13 | Verified |
| 7 | CATTOOR, Lily | SR | Orchard Farm High School | 6:59.06 | Verified |
| 8 | NASH, Kelsey | JR | St. Charles High School | 7:16.76 | Verified |
| 9 | HULBERT, Emma | SR | Orchard Farm High School | 7:19.78 | Verified |
