| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | St. Charles West High School | A | House | Shivers | Watson | Williams | 2:03.63 | Verified |
| 4 | St. Charles High School | A | Kinner | Booker | Oellermann | Starks | 1:57.88 | Verified |
| 5 | Winfield High School | A | Gillespie | Zellmer | Davidson | Bradshaw | 1:58.00 | Verified |
