| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | St. Charles West High School | A | Southard | Litton | Wortham | Blassingame | 8:33.48 | Verified |
| 2 | St. Charles High School | A | Maples | Warner | Case | Bland | 8:46.08 | Verified |
| 3 | Orchard Farm High School | A | VanDyke | Bartig | Reineke | Mobley | 9:27.87 | Verified |
| 5 | Winfield High School | A | Trempler | Ramsour | Geiss | Kaimann | 9:43.18 | Verified |
